EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.369 | expression in Escherichia coli as a C-terminal His6 tag fusion protein | Bacillus subtilis |
5.3.99.11 | expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.369 | 1D-chiro-inositol + NAD+ | Bacillus subtilis | the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA | 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
1.1.1.369 | 1D-chiro-inositol + NAD+ | Bacillus subtilis 168 | the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA | 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.369 | Bacillus subtilis | P26935 | - |
- |
1.1.1.369 | Bacillus subtilis 168 | P26935 | - |
- |
5.3.99.11 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.369 | purified as a C-terminal His6 tag fusion protein | Bacillus subtilis |
5.3.99.11 | - |
Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.369 | 1D-chiro-inositol + NAD+ | the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA | Bacillus subtilis | 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
1.1.1.369 | 1D-chiro-inositol + NAD+ | 1D-chiro-inositol i.e. 1,2,4/3,5,6-cyclohexane-1,2,3,4,5,6-hexol | Bacillus subtilis | 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
1.1.1.369 | 1D-chiro-inositol + NAD+ | the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA | Bacillus subtilis 168 | 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
1.1.1.369 | 1D-chiro-inositol + NAD+ | 1D-chiro-inositol i.e. 1,2,4/3,5,6-cyclohexane-1,2,3,4,5,6-hexol | Bacillus subtilis 168 | 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
5.3.99.11 | 2-oxo-myo-inositol | - |
Bacillus subtilis | 1-oxo-D-chiroinositol | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.369 | iolG | - |
Bacillus subtilis |
5.3.99.11 | inosose isomerase | - |
Bacillus subtilis |
5.3.99.11 | IolI | - |
Bacillus subtilis |